Regulatory

Part:BBa_M36527:Design

Designed by: Valerie Wesley Scott   Group: Stanford BIOE44 - S11   (2015-10-23)

Lactose inducible yeast promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA part was placed into a promoterless version of DNA 2.0's pJ1201 vector (promoterless_pJ1201). It should function well as a promoter so long as it is directly ensued by the kozak sequence and then actuator gene. Also please note that in order for the lactose inducible function of this promoter to work properly, there should be another plasmid in the cell that expresses LacI. Without LacI in the cell, the promoter would likely be working as a constitutive promoter in yeast.

When adapting this promoter from the original BBa_K319019 part, we decided to take out a section towards the end because it mimicked a yeast kozak sequence. The vector that we were putting this promoter into already had the full actuator (immediately following the promoter region) and thus we did not want a repeated kozak section.

Source

Was adapted from the BBa_K319019 part on iGem's Registry of Standard Biological Parts.

References